PUBLICATIONS / CONFERENCE PRESENTATIONS BY RESEARCH AREA

Chromatographic Bioseparation Processes

• Palani S., L. Gueorguieva, U. Rinas, A. Seidel-Morgenstern, G. Jayaraman (2011), Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part I:  Selection of chromatographic system and estimation of adsorption isotherms, Journal of Chromatography A, 1218, 6396-6401

• Gueorguieva L., S. Palani, U. Rinas, G. Jayaraman, A. Seidel-Morgenstern, (2011), Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part II:  Process design and experimental validation, Journal of Chromatography A, 1218, 6402-6411

• Palani S.*, Gueorguieva L., Rinas U., Jayaraman G., Seidel-Morgenstern A., (2010) Simulated Moving Bed Chromatography (SMBC) current status and applications for protein purification (Oral Presn.), International Symposium Gene to Vial Concept for the Biotechnology-based Healthcare Molecules, Feb 7-10, 2010, VIT, Vellore, T.N, India.

• Palani S., Gueorguieva L., Rinas U., Jayaraman G., Seidel-Morgenstern A., (2009) Kontinuierliche aufreinigung der rekombinanten streptokinase mittels simulated moving bed (SMB) chromatographie, (Poster) 27th DECHEMA Jahrestagung der Biotechnologen, Sept 8-10, 2009, Mannheim, Germany.

• Palani S., Gueorguieva L.*, Rinas U., Jayaraman G., Seidel-Morgenstern A., (2009) Determination of adsorption isotherm parameters from total protein mixtures (Oral Presn.),  PREP 2009, July 19-22, 2009, Loews Philadelphia, USA.

• Palani S., Gueorguieva L., Rinas U., Jayaraman G., Seidel-Morgenstern A., (2009) Continuous simulated moving bed (SMB) purification of recombinant streptokinase (Poster), HPLC 2009, June 28-July 2, 2009, Dresden, Germany.

• Palani S.*, Gueorguieva L., Rinas U., Jayaraman G., Seidel-Morgenstern A., (2009) Determination of adsorption isotherm parameters for recombinant streptokinase using perturbation method” (Oral Presn.), 5th Doktorandenseminar Preparative chromatography, Mar 1-3, 2009, Wetter Ruhr, Germany.

• Palani S., G. Jayaraman, L. Gueorguieva, L.C. Keßler, U. Rinas, A. Seidel-Morgenstern (2008), Continuous Separation of Recombinant Streptokinase using HIC-Gradient Simulated Moving Bed Chromatography (Poster), 28th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Sept. 21-24, 2008, Baden-Baden, Germany

• Balagurunathan, B., N.S. Ramchandra, G. Jayaraman (2008), Enhancement of stability of recombinant streptokinase by intracellular expression and single step purification by hydrophobic interaction chromatography, Biochemical Engineering Journal, 39(1), 84-90

• Guhan Jayaraman (1997), Bioseparation Processes under Microgravity Conditions, National Workshop on Materials Processing in Space and Space Biotechnology, August 1, 1997, ISRO, Bangalore

• Cramer, S.M., J. Moore, A. Kundu, Y-F Li and G. Jayaraman (1997), Displacement chromatography of proteins using low molecular weight displacers, Biotechnology Advances, 15(1), 145

• Cramer, S.M., J. Moore, A. Kundu, Y-F Li and G. Jayaraman (1997), Displacement chromatography of proteins using low molecular weight anionic displacers, Biotechnology Advances, 15(3-4), 749

• Kundu, A., S. Vunnum, G. Jayaraman and S.M. Cramer (1995);  Protected amino acids as novel low-molecular-weight displacers in cation-exchange displacement chromatography, Biotechnology and Bioengineering, 48, 452-460

• Jayaraman, G. Y-F Li, J A. Moore and S M. Cramer (1995), Ion-Exchange Displacement Chromatography of Proteins: Dendritic Polymers as Novel Displacers, Journal of Chromatography A, 702 (1-2), 143-156

• Jayaraman, G., Y-F Li, J. A. Moore and S.M. Cramer (1993), Ion-Exchange Displacement Chromatography of Proteins:  Dendritic Polymers as Novel Displacers (Poster), Gordon Conference on Reactive Polymers and Ion-Exchange, Newport, Rhode Island, U.S.A., Aug 23-27, 1993

• Jayaraman, G.* and S.M. Cramer (1993), Purification of Proteins from Complex Biological Mixtures by Displacement Chromatography (Oral Presentation), American Chemical Society 205th Meeting, Denver, Colorado, Mar. 28-April 2, 1993

• Cramer, S.M. and G. Jayaraman (1993), Preparative Chromatography in Biotechnology, Current Opinion in Biotechnology, 4 (2), 217 – 225

• Jayaraman, G., S.D. Gadam and S.M. Cramer (1993), Ion-Exchange Displacement Chromatography of Proteins:  Dextran Based Polyelectrolytes as High Affinity Displacers, Journal of Chromatography A, 630, 53 – 68

• Gadam, S.D., G. Jayaraman and S.M. Cramer (1993), Characterization of Non-Linear Adsorption Properties of Dextran Based Polyelectrolyte Displacers in Ion - Exchange Systems, Journal of Chromatography A, 630, 37-52

• Jayaraman, G. S.D. Gadam and S.M. Cramer (1992), Salt Effects in Ion-Exchange Displacement Chromatography of Proteins (Poster), IX International Biotechnology Symposium, Crystal City, Virginia, U.S.A., Aug. 16 -21, 1992

• Cramer, S.M.* and G. Jayaraman (1991), Chromatographic and Non-Chromatographic Processes for Large-Scale Protein Purification (Keynote Address), American Chemical Society Meeting, New York City, Aug 16 -20, 1991

• Jayaraman, G.*, C.A. Brooks and S.M. Cramer (1990), Ion-Exchange Chromatography of Proteins: Induced Salt Gradient Effects in Displacement Separations (Oral Presentation), AIChE Annual  Meeting 1990, Chicago, Illinois, Nov. 11-16, 1990

• Cramer, S.M.*, G. Subramanian and G. Jayaraman (1989); Novel Displacement Chromatographic Systems (Oral presentation), AIChE Annual Meeting 1989, San Francisco, California, Nov. 15-19, 1989

• Subramanian, G., M. W. Phillips, G. Jayaraman and S. M. Cramer (1989), Displacement Chromatography of Biomolecules in Large Particle Diameter Systems, Journal of Chromatography A, 484, 225 – 236

• Cramer, S.M.*, G. Subramanian, M.W. Philips and G. Jayaraman (1989), Displacement Chromatography of Biomolecules (Oral Presentation), International Conference on Preparative Chromatography, Washington D.C., May 7 -11, 1989

Patents in Process Chromatography

• Cramer,S.M., J.A. Moore, Y. Li, A. Kundu and G. Jayaraman (1995), Displacement Chromatography of Proteins using Low Molecular Weight Displacers, U.S. Patent No. 5,478,924

• Cramer,S.M., G. Jayaraman, A. Kundu, Y. Li and J.A. Moore (1997), Protein Purification at Process Scale by Displacement Chromatography using Low Molecular Weight Anionic Displacers, U.S. Patent No. 5,606,033

Note:  Both the above Patents have been licensed to Sachem Inc., Austin, Texas, USA and have been commercialized.  The low molecular weight displacers from Sachem Inc., which are marketed under the trade names ExpellTM, PropellTM and IsolisTM, are based on the above Patents.

Metabolic Engineering and Systems Biology

• Vaidyanathan H., V. Kandasamy, G.G. Ramakrishnan, K.B. Ramachandran, G. Jayaraman, R. Subramanian (2011), Glycerol conversion to 1,3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri, AMB Express (accepted)

• Sanghe N.*, S.B. Prasad, K.B. Ramachandran, G. Jayaraman (2011), Hyaluronic acid production in metabolically engineered Lactococcus lactis NZ9000: Bioreactor studies and flux balance analysis (Oral Presn.), 1st European Congress of Applied Biotechnology, Sept 25-29, 2011, Berlin, Germany

• Prasad S.B.*, G. Jayaraman, K.B. Ramachandran (2011), Effect of co-expression of has-operon genes on hyaluronic acid production and real-time analysis of their expression in metabolically engineered Lactococcus lactis (Poster), Enzyme Engineering XXI, Sept 18-22, 2011, Vail, Colorado, USA

• Prasad S.B.*, G. Jayaraman, K.B. Ramachandran (2010), Metabolic Engineering of Lactococcus lactis for Hyaluronic Acid Production:  Effect of co-expression of different gene-sets from the has operon (Oral Presn), 14th International Biotechnology Symposium, Sept 14-18, 2010, Rimini, Italy

• Prasad S., G. Jayaraman, K.B. Ramachandran (2010), Hyaluronic acid production is enhanced by the additional coexpression of UDP-glucose Pyrophosphorylase in Lactococcus lactis, Applied Microbiology and Biotechnology, 86, 273-283

• Prasad, S.B.*, G. Jayaraman and K.B. Ramachandran (2008), Production and characterization of hyaluronan by metabolically engineered Lactococcus lactis (Oral Presn.) ChemBiotech'08, Dec. 19-20, 2008, Singapore

• Shashi Bala Prasad, Jagannath S., Guhan Jayaraman, and K.B.Ramachandran (2008), Media optimization for production of hyaluronan by metabolically engineered L.lactis, (Poster), 77th Society for Biological Chemists Conference Dec. 18-21 2008, Chennai

• Jayaraman, G. and B. Balagurunathan (2008), A Systems Biological Approach towards Understanding the Dynamics of Major Heat Shock Proteins on Recombinant Protein Production (Poster), 13th International Biotechnology Symposium, Oct. 12-17, 2008, Dalian, China

• Balagurunathan, B. and G. Jayaraman (2008), Theoretical and experimental investigation of chaperone effects on soluble recombinant proteins in Escherichia coli:  Effect of free DnaK level on temperature-induced recombinant streptokinase production, Systems and Synthetic Biology 2, 27-48

• Guhan Jayaraman (2007), Technological Challenges in the Production of Ethanol from Lignocellulosics (Oral Presn.), Annual Conference of Association of Microbiologists of India, December 18-21, 2007, IIT-Madras, Chennai

• Prasad, S.B., G. Jayaraman and K.B. Ramachandran (2007), Metabolic Engineering of Lactococcus lactis for Production of Hyaluronic Acid(Poster), Annual Conference of Association of Microbiologists of India, December 18-21, 2007, IIT-Madras, Chennai

• Guhan Jayaraman (2005), Cellular Engineering for Industrial Bioprocesses:  Enhancing Productivity through a Systems-Biology Approach, BIOTECH 2005, (Organised by Biotechnology Research Society of India), Gurgaon, Haryana, Dec. 22-24, 2005

• Balagurunathan, B. and G. Jayaraman (2005), Modeling the cellular stress response to temperature induced recombinant protein over expression in Escherichia coli. (Poster), Sixth Annual International Conference on Systems Biology, Boston, October 20-24, 2005

• Balagurunathan, B and G. Jayaraman* (2005), Modeling the regulation of alternative sigma factors s32 and sE during the cellular stress response to temperature induced recombinant protein overexpression (Oral Presentation), Asia-Pacific Biochemical Engineering Conference (APBioChEC 2005), Jeju Island, Korea, May 15 -19, 2005

Recombinant Protein Production

• Jain G., G. Jayaraman, O. Kokpinar, U. Rinas, B. Hitzmann (2011), On-line monitoring of recombinant bacterial cultures using multi-wavelength fluorescent spectroscopy, Biochemical Engineering Journal, 58-59, 133-139

• Karthikeyan T, S. Bera, S. Kalpana, G. Jayaraman* (2010), Effect of Acid Tolerance Response on Recombinant Streptokinase Production in Fed Batch Cultures of Lactococcus lactis P170 Expression System (Poster), 14th International Biotechnology Symposium, Sept 14-18, 2010, Rimini, Italy

• Karthikeyan T.*, S. Bera, G. Jayaraman, (2010) Recombinant protein production in LactococcuslactisP170expression system under acid stress (Oral Presn.), International Symposium Gene to Vial Concept for the Biotechnology-based Healthcare Molecules, Feb 7-10, 2010, VIT, Vellore, T.N, India.

• Jain, G., B. Hitzmann, G. Jayaraman, U. Rinas (2009), Investigation of metabolic changes due to recombinant protein production in E. coli using 2-D fluorescence spectroscopy (Poster) 27th DECHEMA Jahrestagung der Biotechnologen, Sept 8-10, 2009, Mannheim, Germany.

• Jayaraman, G. and B. Balagurunathan (2008), A Systems Biological Approach towards Understanding the Dynamics of Major Heat Shock Proteins on Recombinant Protein Production (Poster), 13th International Biotechnology Symposium, Oct. 12-17, 2008, Dalian, China

• Jain, G. M. Takagie, U. Rinas, G. Jayaraman and B. Hitzmann (2008), Investigation of metabolic changes due to recombinant protein production in E. coli using 2D fluorescence spectroscopy and principal component analysis (Poster), European Bioperspectives, Oct. 5-7, 2008, Hanover, Germany

• Balagurunathan, B. and G. Jayaraman (2008), Theoretical and experimental investigation of chaperone effects on soluble recombinant proteins in Escherichia coli:  Effect of free DnaK level on temperature-induced recombinant streptokinase production, Systems and Synthetic Biology 2, 27-48

• Sriraman, K. and G. Jayaraman (2008), HtrA is essential for efficient secretion of recombinant proteins by Lactococcus lactis, Applied and Environmental Microbiology 74(23), 7442-7446

• Balagurunathan, B. and G. Jayaraman (2008), Cellular response to accumulation of recombinant proteins in the E. coli inner membrane: Implications for proteolysis and productivity of the secretory expression system, Biochemical Engineering Journal, 39(1), 74-83

• Balagurunathan, B., N.S. Ramchandra, G. Jayaraman (2008), Enhancement of stability of recombinant streptokinase by intracellular expression and single step purification by hydrophobic interaction chromatography, Biochemical Engineering Journal, 39(1), 84-90

• Bera, S. and G. Jayaraman (2007), Development of Bioprocess Strategies for Enhancing Production of Recombinant Streptokinase in Lactococcus lactis MG1363 (Poster), Annual Conference of Association of Microbiologists of India, December 18-21, 2007, IIT-Madras, Chennai

• Balagurunathan, B. and G. Jayaraman (2007), Dynamics of cellular response to recombinant protein overexpression in Escherichia coli, in G. Subramanian (Ed.), Bioseparation and Bioprocessing: A Handbook (2nd Ed., Wiley VCH), Vol. 1, 101-122

• Ramalingam, S., P. Gautam, K.J. Mukherjee, G. Jayaraman (2007), Effects of post-induction feed strategies on secretory production of recombinant streptokinase in Escherichia coli, Biochemical Engineering Journal, 33(1), 34-41

• Sriraman, K. and G. Jayaraman (2006), Enhancement of recombinant streptokinase production in Lactococcus lactis by suppression of acid tolerance response, Applied Microbiology and Biotechnology, 72(6), 1202–1209

• Balagurunathan, B and G. Jayaraman (2005), Kinetic analysis of the envelope stress response during the temperature induced periplasmic expression of recombinant streptokinase in Escherichia coli. (Poster), AIChE Annual Meeting 2005, Cincinnati, Ohio

• Balagurunathan, B. and G. Jayaraman (2005), Modeling the cellular stress response to temperature induced recombinant protein over expression in Escherichia coli. (Poster), Sixth Annual International Conference on Systems Biology, Boston, October 20-24, 2005

• Balagurunathan, B and G. Jayaraman* (2005), Modeling the regulation of alternative sigma factors s32 and sE during the cellular stress response to temperature induced recombinant protein overexpression (Oral Presentation), Asia-Pacific Biochemical Engineering Conference (APBioChEC 2005), Jeju Island, Korea, May 15 -19, 2005

• Balagurunathan, B. and G. Jayaraman (2005), Improvement of Productivity of Recombinant Streptokinase in Escherichia coli by Co-expression of Molecular Chaperones (Poster) Asia-Pacific Biochemical Engineering Conference (APBioChEC 2005), Jeju Island, Korea.

• Kalpana, S. and G. Jayaraman (2005), Expression Studies of Recombinant Streptokinase in Lactococcus lactis (Poster), Asia-Pacific Biochemical Engineering Conference (APBioChEC 2005), Jeju Island, Korea, May 15 -19, 2005

• Kalpana, S.* and G. Jayaraman (2003), Production of Recombinant Proteins in Lactococcus lactis (Oral Presentation), Indian Chemical Engineering Congress (CHEMCON 2003), Regional Research Laboratory, Bhuvaneshwar, Orissa , Dec. 2003

• Velu, M.*, S. Ramalingam and G. Jayaraman (2003), Model-based development of a fed-batch process for recombinant streptokinase production (Oral Presentation), Indian Chemical Engineering Congress (CHEMCON 2003), Regional Research Laboratory, Bhuvaneshwar, Orissa, Dec. 2003

• Balagurunathan, B.* and G. Jayaraman (2003), Modeling of cellular stress response to recombinant protein production in bacterial systems (Oral Presentation), Indian Chemical Engineering Congress (CHEMCON- 2003), Regional Research Laboratory, Bhuvaneshwar, Orissa, Dec. 2003

• Jayaraman, G* and R. Mathangi (1998), Effect of Carbon and Nitrogen Components on Growth, Sporulation and Crystal Toxin Production by Bacillus thuringiensis var. galleriae, National Symposium on Microbial Pesticides, Pune, Nov. 12-13, 1998

Bioprocess Monitoring and Control

• Jain G., G. Jayaraman, O. Kokpinar, U. Rinas, B. Hitzmann (2011), On-line monitoring of recombinant bacterial cultures using multi-wavelength fluorescent spectroscopy, Biochemical Engineering Journal, 58-59, 133-139

• Velu, M.*, S. Ramalingam and G. Jayaraman (2005), Extended Kalman filter based monitoring of recombinant streptokinase production (Oral presentation), Indian Chemical Engineering Congress (CHEMCON-2005), Dec. 16-20 2005, IIT Delhi, India.

• Velu, M., B. Balagurunathan* and G. Jayaraman (2005), Online performance monitoring of recombinant protein production in a fed-batch reactor. (Oral Presentation), AIChE Annual Meeting 2005, Cincinnati, Ohio, Oct 31 -Nov 4, 2005

• Velu, M., S.Ramalingam and G. Jayaraman (2005), Modelling and control of fed-batch processes for proteolytically sensitive recombinant proteins:  A case-study with recombinant streptokinase (Poster), Biochemical Engineering XIV, June 2005, Harrison Hot Springs, Canada, June 10-14, 2005

• Velu, M., S. Ramalingam and G. Jayaraman (2005), Modelling and control of fed-batch process for a proteolytically sensitive recombinant protein (Poster), Asia Pacific Biochemical Engineering Conference (APBioChEC 2005), Jeju Island, Korea, May 15 -19, 2005

• Vellanki, P., I. Marison, G. Jayaraman and K. Jayaraman (1998), Calorimetric optimisation of growth and sporulation of Bacillus thuringiensis var galleriae, Thermochimica Acta , 309, 105 – 110

• Vellanki, P. I. Marison, G. Jayaraman, and K. Jayaraman (1997) Heat measurement and its application in the optimisation of biopesticide production by Bacillus thuringiensis (Poster), International Symposium on Biological Calorimetry, Switzerland, April 28-30, 1997

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