Hematopoiesis is an exquisitely regulated physiological process, which results in the appropriate production of blood cells throughout life. Over the past four decades our understanding of the cellular and molecular basis of hematopoietic regulation has become quite sophisticated. Several hematopoietic growth factor polypeptide hormones have been identified through molecular cloning, which play a key role in this process. In many respect, our current dynamic model of hematopoietic is the leading paradigm in molecular histology. It has established a framework to apply to cell differentiation and renewal in diverse tissue, including liver, skin and intestinal epithelium. Despite these advances, the most important details of the cellular and molecular regulation of stem cell self- renewal and differentiation remain elusive. We are currently addressing these issues through the following:

1. Role of Hematopoietic permissive and inductive microenvironment in bone marrow in context to proliferation of stem cells

2. Role of osteoblast in the proliferation of stem cell

3. Construction of an expression vector which could be used in the expression of cloned cDNA in lower eukaryotic cells, as this may facilitate the large production of purified protein for further characterization.

4. Cloning and expression of four therapeutic proteins which are of clinical importance for commercial usages

5. Developing a molecular assay system for the new drug discovery research.

6. Characterization of membrane proteins (receptors) at a biochemical and molecular level. Presently my interest is in “Identification and Purification of Methotrexate Binding protein from the T Lymphocytes”