Research



1 -Trans-differentiation of stem cell into cardiac tissue

To asses the trans-differentiation potential of Adult Stem cell into Cardiomyocyte, this work uses Mouse bone marrow derived stem cells (BMSC) and allows them to differentiate into cells of cardiomyogenic lineage in presence of differentiation inducing factors like 5-Azacytidine (5-azaC) & Niche. We are using a biomaterial scaffold - Cardiogel, to imitate in vivo niche conditions of the Heart. Cardiogel along with 5-azaC has been found to increase differentiation of cells in cardiomyogenic lineage. Cardiogel also shows stress protection ability against Oxidative stress. BMSC growing on cardiogel showed increased proliferation, faster adherence and resistance to enzymatic dissociation. Cardiogel can be used as a delivery material for stem cells in Cardiac regeneration therapy though its tissue engineering abilities is yet to be harnessed. In order to generate Venticular Cardiomyogenic Cell lines, we have generated Mouse Mesenchymal stem cell lines, which are now being characterized. Besides these we have also standardized a faster & cost efficient protocol for cardiomyocyte isolation and culture.

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2 - Construction of Novel Immunotoxins

Immunotoxins are chimeric proteins with a cytotoxic protein linked to a cell-targeting moiety such as a cytokine or monoclonal antibody. Immunotoxins offer means for selective targeting and elimination of cancer cells over-expressing specific cell surface receptors. After binding to target cells, these proteins are internalized by receptor -mediated endocytosis and cell death is induced by the toxin moiety.

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3 - Fanconi Anemia

Fanconi anemia (FA) is a genetic disorder characterized by progressive bone marrow failure and a predisposition to cancers like acute myeloid leukemia, lung and squamous cell carcinomas. DNA damage in a healthy cell activates the FA pathway where 15 individual FA proteins interact and function together to maintain genomic stability.

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4 - Development of Monoclonal antibodies for targeted therapy

Epithelial cell adhesion (EpCAM) has gained interest as a potential therapeutic target and is an attractive candidate tumor-associated antigen (TAA) for antibody-based immunotherapy. The correlation between EpCAM expression and prognosis seems to be tissue specific.

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5 - Folate receptor a model for targeted approach for cancer

Methotrexate has been used as an effective anticancer drug for a long time. Conceptually it is accepted that methotrexate (MTX) and folic acid (FA) are transported by folate receptors in cancerous cells, but the exact mechanism of MTX uptake in human leukemia is unknown.

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6 - Elucidation of various pathways in cancer model using plant oil's

New agents with novel mechanisms of action to prevent cancer are perhaps the most urgent need in the entire field of chemoprevention.

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7 - Stem cell biologyand Tissue regeneration

Differentiation of Stem cells into different lineages has immense potential in cell replacement therapy. Our group has focused on improving rates of differentiation of adult stem cells using 3-Dimensional culture systems, improving isolation of pure populations of stem cells from bone marrow and other sources such as Umbilical cord blood and the Heart.

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8 - Molecular Basis of Fanconi anemia

Fanconi anemia (FA) is a genetic disorder characterized by progressive bone marrow failure and a predisposition to cancers like acute myeloid leukemia, lung and squamous cell carcinomas. DNA damage in a healthy cell activates the FA pathway where 15 individual FA proteins interact and function together to maintain genomic stability.

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9 - Targeted Cancer therapy

Targeted therapy of cancer has shown considerable promise in treating cancer with the increased understanding of their molecular mechanisms. Immunotoxins are chimeric fusion proteins that selectively targets and destroys cancer cells.

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