LC-MS (Orbitrap Elite)

 

Mass Spectrometry Service

Orbitrap Elite:

Thermo Scientific Orbitrap Elite Mass spectrometer has a dual pressure linear ion trap coupled with high-field Orbitrap Mass analyzer facilitating increased sensitivity, reliability and dynamic range for better detection and MSn identification.

Features:

  • Novel high-field Orbitrap analyzer
  • Resolving power > 240,000 FWHM
  • Advanced signal processing
  • Multiple fragmentation techniques: CID, HCD and optionally ETD
  • Parallel MS and MSn analysis
  • Velos Pro ion trap generation II ion optics with neutral blocking technology increase robustness

 

Small Molecules:

Molecules ranging from 100 to 2000 Da can be analyzed for their presence through direct infusion method with an accuracy of < 5 ppm in high resolution mode.

 

External Academic External Industries
Service Charge 1000+18%(Service Tax)* 3000+18%(Service Tax)
(Small Molecules Form for Internal)  https://biotech.iitm.ac.in/Internal_Mass form_Small Molecules (Small Molecules Form for External)                      (click here for slot checking)

Top Down Proteomics:

This involves identification of proteins present in a complex mixture prior digestion.  Proteins extracted from the sample of interest and fractionated can be analysed using Orbitrap based Mass spectrometer with CID, HCD and ETD fragmentation.

External Academic External Industries
Service Charge 10000+18%(Service Tax)* 20000+18%(Service Tax)

Bottom Up Proteomics:

Digested Protein samples can be analysed by LC-MS/MS. Peptides are separated using a nano LC-Column and the eluent is subjected to Mass spectrometer using Electrospray ionization.

External Academic External Industries
Service Charge 10000+18%(Service Tax)* 20000+18%(Service Tax)

Targeted Quantification:

Mass of the target is monitor in the MS1 level and MS/MS scans can be triggered on confirmation of the target.

External Academic External Industries
Service Charge 10000+18%(Service Tax)* 20000+18%(Service Tax)

Intact Protein Characterization:

Purified intact proteins can be characterized by top down methodology in an Orbitrap Elite Mass spectrometer by either direct infusion or LC coupled to an ESI source.

External Academic External Industries
Service Charge 10000+18%(Service Tax)* 20000+18%(Service Tax)
(Proteomics Form for Internal)               (Proteomics Form for External)                      (click here for slot checking)

* Service Tax is exempted for samples within IITM.

For sample submission and slot booking contact : orbitrapelite.iitm@gmail.com