Mass Spectrometry Service
Orbitrap Elite:
Thermo Scientific Orbitrap Elite Mass spectrometer has a dual pressure linear ion trap coupled with high-field Orbitrap Mass analyzer facilitating increased sensitivity, reliability and dynamic range for better detection and MSn identification.
Features:
- Novel high-field Orbitrap analyzer
- Resolving power > 240,000 FWHM
- Advanced signal processing
- Multiple fragmentation techniques: CID, HCD and optionally ETD
- Parallel MS and MSn analysis
- Velos Pro ion trap generation II ion optics with neutral blocking technology increase robustness
Small Molecules:
Molecules ranging from 100 to 2000 Da can be analyzed for their presence through direct infusion method with an accuracy of < 5 ppm in high resolution mode.
External Academic | External Industries | |
Service Charge | 1000+18%(Service Tax)* | 3000+18%(Service Tax) |
(Small Molecules Form for Internal) https://biotech.iitm.ac.in/Internal_Mass form_Small Molecules | (Small Molecules Form for External) | (click here for slot checking) |
Top Down Proteomics:
This involves identification of proteins present in a complex mixture prior digestion. Proteins extracted from the sample of interest and fractionated can be analysed using Orbitrap based Mass spectrometer with CID, HCD and ETD fragmentation.
External Academic | External Industries | |
Service Charge | 10000+18%(Service Tax)* | 20000+18%(Service Tax) |
Bottom Up Proteomics:
Digested Protein samples can be analysed by LC-MS/MS. Peptides are separated using a nano LC-Column and the eluent is subjected to Mass spectrometer using Electrospray ionization.
External Academic | External Industries | |
Service Charge | 10000+18%(Service Tax)* | 20000+18%(Service Tax) |
Targeted Quantification:
Mass of the target is monitor in the MS1 level and MS/MS scans can be triggered on confirmation of the target.
External Academic | External Industries | |
Service Charge | 10000+18%(Service Tax)* | 20000+18%(Service Tax) |
Intact Protein Characterization:
Purified intact proteins can be characterized by top down methodology in an Orbitrap Elite Mass spectrometer by either direct infusion or LC coupled to an ESI source.
External Academic | External Industries | |
Service Charge | 10000+18%(Service Tax)* | 20000+18%(Service Tax) |
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* Service Tax is exempted for samples within IITM.
For sample submission and slot booking contact : orbitrapelite.iitm@gmail.com