Protein Biophysics Lab

pStab web server provides a robust and fast strategy to engineer protein stabilities through mutations involving charged residues.

We expect the method to be of importance:

• As a first and rapid step to screen for protein mutants with specific stability in the biotechnology industry.
• In the construction of stability maps at the residue level (i.e., hot spots).
• As a robust tool to probe for mutations that enhance the stability of protein-based drugs.
• To explore the role of electrostatic frustration in proteins.

pPerturb web server provides a rapid and experimentally consistent measure of the extent to which a particular residue is coupled to its neighbors.

We expect the method to be of importance:

• To explore the strength of the interaction network around a particular residue.
• In quantifying the partitioning of destabilization energetics around the mutation neighbourhood.
• As a simple theoretical framework for modelling allosteric effects.
• To explore the effects of thermodynamics upon side-chain truncating mutations.