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Guhan Jayaraman

guhan-jayaraman
Professor
Ph.D, Rensselaer Polytechnic Institute
M.Tech, Indian Institute of Technology Delhi
Office: BT 501
Tel: +91-44-2257-4108
Email: guhanj@iitm·ac·in
Lab: BT 216 (Website)

Research Interests

  • Metabolic engineering and systems biology
  • Recombinant protein production
  • Bioprocess monitoring and control
  • Chromatographic bioseparation processes

Recent Publications/ Presentation

• Jain G., G. Jayaraman, O. Kokpinar, U. Rinas, B. Hitzmann (2011), On-line monitoring of recombinant bacterial cultures using multi-wavelength fluorescent spectroscopy, Biochemical Engineering Journal, 58-59, 133-139

• Palani S., L. Gueorguieva, U. Rinas, A. Seidel-Morgenstern, G. Jayaraman (2011), Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part I: Selection of chromatographic system and estimation of adsorption isotherms, Journal of Chromatography A, 1218, 6396-6401

• Gueorguieva L., S. Palani, U. Rinas, G. Jayaraman, A. Seidel-Morgenstern, (2011), Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part II: Process design and experimental validation, Journal of Chromatography A, 1218, 6402-6411

• Sanghe N.*, S.B. Prasad, K.B. Ramachandran, G. Jayaraman (2011), Hyaluronic acid production in metabolically engineered Lactococcus lactis NZ9000: Bioreactor studies and flux balance analysis (Oral Presn.), 1st European Congress of Applied Biotechnology, Sept 25-29, 2011, Berlin, Germany

• Prasad S.B.*, G. Jayaraman, K.B. Ramachandran (2011), Effect of co-expression of has-operon genes on hyaluronic acid production and real-time analysis of their expression in metabolically engineered Lactococcus lactis (Poster), Enzyme Engineering XXI, Sept 18-22, 2011, Vail, Colorado,USA

2010

• Prasad S., G. Jayaraman, K.B. Ramachandran (2010), Hyaluronic acid production is enhanced by the additional coexpression of UDP-glucose Pyrophosphorylase in Lactococcus lactis, Applied Microbiology and Biotechnology, 86, 273-283

• Prasad S.B.*, G. Jayaraman, K.B. Ramachandran (2010), Metabolic Engineering of Lactococcus lactis for Hyaluronic Acid Production: Effect of co-expression of different gene-sets from the has operon (Oral Presn), 14th International Biotechnology Symposium, Sept 14-18, 2010, Rimini, Italy

• Karthikeyan T, S. Bera, S. Kalpana, G. Jayaraman* (2010), Effect of Acid Tolerance Response on Recombinant Streptokinase Production in Fed Batch Cultures of Lactococcus lactis P170 Expression System (Poster), 14th International Biotechnology Symposium, Sept 14-18, 2010, Rimini, Italy

• Karthikeyan T.*, S. Bera, G. Jayaraman, (2010) Recombinant protein production in LactococcuslactisP170expression system under acid stress (Oral Presn.), International Symposium Gene to Vial Concept for the Biotechnology-based Healthcare Molecules, Feb 7-10, 2010, VIT, Vellore, T.N, India.

• Palani S.*, Gueorguieva L., Rinas U., Jayaraman G., Seidel-Morgenstern A., (2010) Simulated Moving Bed Chromatography (SMBC) current status and applications for protein purification (Oral Presn.), International Symposium Gene to Vial Concept for the Biotechnology-based Healthcare Molecules, Feb 7-10, 2010, VIT, Vellore, T.N, India.

2009

• Jain, G., B. Hitzmann, G. Jayaraman, U. Rinas (2009), Investigation of metabolic changes due to recombinant protein production in E. coli using 2-D fluorescence spectroscopy (Poster) 27th DECHEMA Jahrestagung der Biotechnologen, Sept 8-10, 2009, Mannheim, Germany.

• Palani S., Gueorguieva L., Rinas U., Jayaraman G., Seidel-Morgenstern A., (2009) Kontinuierliche aufreinigung der rekombinanten streptokinase mittels simulated moving bed (SMB) chromatographie, (Poster) 27th DECHEMA Jahrestagung der Biotechnologen, Sept 8-10, 2009, Mannheim, Germany.

• Palani S., Gueorguieva L.*, Rinas U., Jayaraman G., Seidel-Morgenstern A., (2009) Determination of adsorption isotherm parameters from total protein mixtures (Oral Presn.), PREP 2009, July 19-22, 2009, Loews Philadelphia, USA.

• Palani S., Gueorguieva L., Rinas U., Jayaraman G., Seidel-Morgenstern A., (2009) Continuous simulated moving bed (SMB) purification of recombinant streptokinase (Poster), HPLC 2009, June 28-July 2, 2009, Dresden, Germany.

• Palani S.*, Gueorguieva L., Rinas U., Jayaraman G., Seidel-Morgenstern A., (2009) Determination of adsorption isotherm parameters for recombinant streptokinase using perturbation method” (Oral Presn.), 5th Doktorandenseminar Preparative chromatography, Mar 1-3, 2009, Wetter Ruhr, Germany.

2008

• Sriraman, K. and G. Jayaraman (2008), HtrA is essential for efficient secretion of recombinant proteins by Lactococcus lactis, Applied and Environmental Microbiology 74(23), 7442-7446

• Balagurunathan, B. and G. Jayaraman (2008), Theoretical and experimental investigation of chaperone effects on soluble recombinant proteins in Escherichia coli: Effect of free DnaK level on temperature-induced recombinant streptokinase production, Systems and Synthetic Biology 2, 27-48

• Balagurunathan, B. and G. Jayaraman (2008), Cellular response to accumulation of recombinant proteins in the E. coli inner membrane: Implications for proteolysis and productivity of the secretory expression system, Biochemical Engineering Journal, 39(1), 74-83

• Balagurunathan, B., N.S. Ramchandra, G. Jayaraman (2008), Enhancement of stability of recombinant streptokinase by intracellular expression and single step purification by hydrophobic interaction chromatography, Biochemical Engineering Journal, 39(1), 84-90

• Prasad, S.B.*, G. Jayaraman and K.B. Ramachandran (2008), Production and characterization of hyaluronan by metabolically engineered Lactococcus lactis (Oral Presn.) ChemBiotech’08, Dec. 19-20, 2008, Singapore

• Shashi Bala Prasad, Jagannath S., Guhan Jayaraman, and K.B.Ramachandran (2008), Media optimization for production of hyaluronan by metabolically engineered L.lactis, (Poster), 77th Society for Biological Chemists Conference Dec. 18-21 2008, Chennai

• Jayaraman, G. and B. Balagurunathan (2008), A Systems Biological Approach towards Understanding the Dynamics of Major Heat Shock Proteins on Recombinant Protein Production (Poster), 13th International Biotechnology Symposium, Oct. 12-17, 2008, Dalian, China

• Jain, G. M. Takagie, U. Rinas, G. Jayaraman and B. Hitzmann (2008), Investigation of metabolic changes due to recombinant protein production in E. coli using 2D fluorescence spectroscopy and principal component analysis (Poster), European Bioperspectives, Oct. 5-7, 2008, Hanover, Germany

• Palani S., G. Jayaraman, L. Gueorguieva, L.C. Keßler, U. Rinas, A. Seidel-Morgenstern (2008), Continuous Separation of Recombinant Streptokinase using HIC-Gradient Simulated Moving Bed Chromatography (Poster), 28th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Sept. 21-24, 2008, Baden-Baden, Germany

2007

• Balagurunathan, B. and G. Jayaraman (2007), Dynamics of cellular response to recombinant protein overexpression in Escherichia coli, in G. Subramanian (Ed.), Bioseparation and Bioprocessing: A Handbook (2nd Ed., Wiley VCH), Vol. 1, 101-122

• Ramalingam, S., P. Gautam, K.J. Mukherjee, G. Jayaraman (2007), Effects of post-induction feed strategies on secretory production of recombinant streptokinase in Escherichia coli, Biochemical Engineering Journal, 33(1), 34-41

• Guhan Jayaraman (2007), Technological Challenges in the Production of Ethanol from Lignocellulosics (Oral Presn.), Annual Conference of Association of Microbiologists of India, December 18-21, 2007, IIT-Madras, Chennai

• Prasad, S.B., G. Jayaraman and K.B. Ramachandran (2007), Metabolic Engineering of Lactococcus lactis for Production of Hyaluronic Acid (Poster), Annual Conference of Association of Microbiologists of India, December 18-21, 2007, IIT-Madras, Chennai

• Bera, S. and G. Jayaraman (2007), Development of Bioprocess Strategies for Enhancing Production of Recombinant Streptokinase in Lactococcus lactis MG1363 (Poster), Annual Conference of Association of Microbiologists of India, December 18-21, 2007, IIT-Madras, Chennai