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Faculty

Guhan Jayaraman

Guhan Jayaraman

Ph.D, Rensselaer Polytechnic Institute

M.Tech, Indian Institute of Technology Delhi

Professor

guhanj@iitm.ac.in

Office : BT 501 Block _ 1

+91-44-2257-4108

Research Interests

  • Metabolic engineering and systems biology

  • Recombinant protein production

  • Bioprocess monitoring and control

  • Chromatographic bioseparation processes

Recent Publications

  1. Muthukrishnan, A.B., G. Jayaraman, A. Hakkinen, V.D. Rajendran, A. Kozhiyalam (2020), In vivo single-cell analysis using calcofluor - white staining detects high expression phenotype in L. lactis cultures engineered for hyaluronic acid production, bioRxiv

  2. Vishnu Prasad J., T.K. Sahoo, S. Naveen, G. Jayaraman (2020), Evolutionary engineering of Lactobacillus bulgaricus reduces enzyme usage and enhances conversion of lignocellulosics to D-lactic acid by simultaneous saccharification and fermentation, Biotechnology for Biofuels, 13(1):171, DOI: 10.1186/s13068-020-01812-x

  3. Agarwal G., K.V Krishnan , S.B. Prasad , A. Bhaduri, G. Jayaraman (2019), Biosynthesis of Hyaluronic acid polymer: Dissecting role of sub structural elements of hyaluronan synthase, Scientific Reports , 9:12510 (doi: 1038/s41598-019-48878-8)

  4. Richa Srivastava, Anantha Barathi Muthukrishnan* & Guhan Jayaraman* (2019) Design and construction of a synthetic riboregulator-based platform for metabolic shunting of pathways in Lactococcus lactis, **Proceedings of the Singapore National Academy of Science,** V.1(1), 1-10 (in Press; DOI: 10.1142/S2591722619400027)

  5. Kailash B P., Karthik D., Mousami Shinde, Nikhita Damaraju, Anantha Barathi Muthukrishnan, Shashi Bala Prasad, Guhan Jayaraman (2019), ChassiDex: A microbial database useful for synthetic biology applications, bioRxiv doi

  6. Schulte S. , S. Shanmuga Doss , P. Jeeva; M. Ananth, L. M. Blank, G. Jayaraman (2019), Exploiting the diversity of streptococcal hyaluronan synthases for the production of molecular weight-tailored hyaluronan, Applied Microbiology and Biotechnology,** 103, 7567-7581

  7. Puvendran K. and G. Jayaraman (2019), Enhancement of acetyl-CoA by acetate co-utilization in recombinant Lactococcus lactis cultures enables the production of high molecular weight hyaluronic acid, ** Applied Microbiology and Biotechnology,** 103, 6989-7001

  8. Badri A., K. Raman *, G. Jayaraman * (2019),Uncovering novel pathways for enhancing hyaluronan synthesis in recombinant Lactococcus lactis: Genome-scale metabolic modelling and experimental validation, Processes, 7(6), 343 (doi:10.3390/pr7060343) bioRxiv

  9. Sahoo T.K. and G. Jayaraman (2019), Co-Culture of Lactobacillus delbrueckii and Engineered Lactococcus lactis enhances stoichiometric yield of D-Lactic Acid from whey permeate, Applied Microbiology and Biotechnology , 103, 5653-5662

  10. Jeeva P., S. Shanmuga Doss, V. Sundaram, G. Jayaraman (2019), Production of controlled molecular weight hyaluronic acid by glucostat strategy using recombinant Lactococcus lactis cultures, Applied Microbiology and Biotechnology , 103, 4363-4375

  11. Puvendran K., K. Anupama, G. Jayaraman (2018), Real time monitoring of hyaluronic acid fermentation by in-situ transflectance spectroscopy,** Applied Microbiology and Biotechnology ,** 102, 2659-2669

  12. Shanmuga Doss S., N.P. Bhatt, G. Jayaraman (2017), Improving the accuracy of hyaluronic acid molecular weight estimation by conventional size exclusion chromatography, Journal of Chromatography B , 1060, 255 - 261

  13. Kaur M. and G. Jayaraman (2016), Hyaluronan Production and Molecular Weight is Enhanced in Pathway-Engineered Strains of Lactate Dehydrogenase-Deficient Lactococcus lactis, Metabolic Engineering Communications, 3, 15-23

  14. Rajendran V., K. Puvendran, Bharath Raja Guru, G. Jayaraman (2016), Design of aqueous two-phase systems for purification of hyaluronic acid produced by metabolically engineered Lactococcus lactis,** Journal of Separation Science,** 39, 655-662

  15. Bera , K. Thillai, K. Sriraman, G. Jayaraman (2015), Process strategies for enhancing recombinant streptokinase production in Lactococcus lactis cultures using P170 expression system, Biochemical Engineering Journal, 93, 94-101

  16. Badri, A., K. Raman, Jayaraman (2015), Investigation of metabolic capabilities of recombinant Lactococcus lactis for production of hyaluronic acid using constraint based genome scale models, Proceedings of 5th International Conference on Biomolecular Engineering 2015, 85-87

  17. Hmar R.V., S.B. Prasad, Jayaraman*, K. B. Ramachandran* (2014), Chromosomal integration of hyaluronic acid synthesis (has) genes enhances the molecular weight of hyaluronan produced in Lactococcus lactis,**Biotechnology Journal,** 9, 1554-1564

  18. Chauhan A., Badle S.S., K.B. Ramachandran, G. Jayaraman (2014) The P170 expression system enhances hyaluronan molecular weight and production in metabolically-engineered Lactococcus lactis ,Biochemical Engineering Journal, 90, 73-78

  19. Badle S.S., G. Jayaraman, K.B. Ramachandran (2014) Ratio of intracellular precursors concentration and their flux influences hyaluronic acid molecular weight in Streptococcus zooepidemicus and recombinant Lactococcus lactis, Bioresource Technology, 163, 222-227

  20. Baikadi A.K., M. Kaur, S. Sreeja, G. Jayaraman, S. Narasimhan (2013), Extraction of pure component spectrum from mixture spectra containing a known diluent, 10th IFAC Proceedings, Vol. 10, 649-653

  21. Kandasamy V., H. Vaidyanathan, I. Djurdjevic, E. Jayamani, K.B. Ramachandran, W. Buckel, Jayaraman, S. Ramalingam (2013) Engineering Escherichia coli with acrylate pathway genes for propionic acid synthesis and its impact on mixed-acid fermentation, Applied Microbiology and Biotechnology, 97(3), 1191-1200

  22. Prasad S., K.B. Ramachandran, G. Jayaraman (2012), Transcription analysis of hyaluronan biosynthesis genes in Streptococcus zooepidemicus and metabolically engineered Lactococcus lactis,Applied Microbiology and Biotechnology, 94: 1593-1607

  23. Vaidyanathan H., V. Kandasamy, G.G. Ramakrishnan, KB Ramachandran, G. Jayaraman, S. Ramalingam (2011), Glycerol conversion to 1,3 - Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri, AMB Express, 1:37

  24. Jain G., G. Jayaraman, O. Kokpinar, U. Rinas, B. Hitzmann (2011), On-line monitoring of recombinant bacterial cultures using multi-wavelength fluorescent spectroscopy, Biochemical Engineering Journal, 58-59, 133-139

  25. Palani S., Gueorguieva, U. Rinas, A. Seidel-Morgenstern, G. Jayaraman (2011), Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part I: Selection of chromatographic system and estimation of adsorption isotherms, Journal of Chromatography A, 1218, 6396-6401

  26. Gueorguieva, S. Palani, U. Rinas, G. Jayaraman, A. Seidel-Morgenstern, (2011), Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part II: Process design and experimental validation, Journal of Chromatography A, 1218, 6402-6411

  27. Prasad S., G. Jayaraman, K.B. Ramachandran (2010), Hyaluronic acid production is enhanced by the additional coexpression of UDP-glucose Pyrophosphorylase in Lactococcus lactis, Applied Microbiology and Biotechnology, 86, 273-283

  28. Palani S., Jayaraman, L. Gueorguieva, U. Rinas, A. Seidel-Morgenstern (2009), Kontinuierliche Aufreinigung der rekombinanten Streptokinasemittels Simulated Moving Bed (SMB)-Chromatographie, Chemie Ingenieur Technik, 81(8), P11.35, 1291

  29. Jain G., B. Hitzmann, U. Rinas, G. Jayaraman (2009), 2D-Fluoreszenzspektroskopie zur Stressanalyse bei der rekombinanten Proteinproduktion, Chemie Ingenieur Technik, 81(8), P11.37, 1292

  30. Balagurunathan, B. and G. Jayaraman (2008), Theoretical and experimental investigation of chaperone effects on soluble recombinant proteins in Escherichia coli: Effect of free DnaK level on temperature-induced recombinant streptokinase production, Systems and Synthetic Biology 2, 27-48

  31. Sriraman, K. and G. Jayaraman (2008), HtrA is essential for efficient secretion of recombinant proteins by Lactococcus lactis, Applied and Enviromental Microbiology 74(23), 7442-7446

  32. Balagurunathan, B. and G. Jayaraman (2008), Cellular response to accumulation of recombinant proteins in the coli inner membrane: Implications for proteolysis and productivity of the secretory expression system, Biochemical Engineering Journal, 39, 74-83

  33. Balagurunathan, B., N.S. Ramchandra, G. Jayaraman (2008), Enhancement of stability of recombinant streptokinase by intracellular expression and single step purification by hydrophobic interaction chromatography, Biochemical Engineering Journal 39(1), 84-90

  34. Balagurunathan, B. and G. Jayaraman (2007), Dynamics of cellular response to recombinant protein overexpression in Escherichia coli, in G. Subramanian (Ed.), Bioseparation and Bioprocessing: A Handbook (2nd, Wiley VCH), Vol. 1, 101-122

  35. Ramalingam, S., P. Gautam, K.J. Mukherjee, G. Jayaraman (2007), Effects of post-induction feed strategies on secretory production of recombinant streptokinase in Escherichia coli, Biochemical Engineering Journal 33(1), 34-41

  36. Sriraman, K. and G. Jayaraman (2006), Enhancement of recombinant streptokinase production in Lactococcus lactis by suppression of acid tolerance response, Applied Microbiology and Biotechnology, 72(6), 1202-1209

  37. Balagurunathan, G. Jayaraman (2005), Kinetic analysis of the envelope stress response during the temperature induced periplasmic expression of recombinant streptokinase in escherichia coli, AIChE Annual Meeting 2005, Conference Proceedings, 6297-6298

  38. Velu, M., B. Balagurunathan, G. Jayaraman (2005), Online performance monitoring of recombinant protein production in a fed-batch bioreactor, AIChE Annual Meeting 2005, Conference Proceedings, 8445

  39. Balagurunathan, B. and G. Jayaraman (2003), Modeling of cellular stress response to recombinant protein production in bacterial systems, Proceedings of the Indian Chemical Engineering Congress 2003

  40. Vellanki, P., I. Marison, G. Jayaraman and K. Jayaraman (1998), Calorimetric optimisation of growth and sporulation of Bacillus thuringiensis var galleriae, Thermochimica Acta , 309, 105 - 110

  41. Cramer, S.M., J. Moore, A. Kundu, Y-F Li and G. Jayaraman (1997), Displacement chromatography of proteins using low molecular weight displacers, Biotechnology Advances, 15(1), 145

  42. Cramer, S.M., J. Moore, A. Kundu, Y-F Li and G. Jayaraman (1997), Displacement chromatography of proteins using low molecular weight anionic displacers, Biotechnology Advances, 15(3-4), 749

  43. Kundu, A., S. Vunnum, G. Jayaraman and S.M. Cramer (1995); Protected amino acids as novel low-molecular-weight displacers in cation-exchange displacement chromatography, Biotechnology and Bioengineering, 48, 452-460

  44. Jayaraman, G. Y-F Li, J A. Moore and S M. Cramer (1995), Ion-Exchange Displacement Chromatography of Proteins: Dendritic Polymers as Novel Displacers, Journal of Chromatography A, 702 (1-2), 143-156

  45. Cramer, S.M. and G. Jayaraman (1993), Preparative Chromatography in Biotechnology, Current Opinion in Biotechnology, 4 (2), 217 - 225

  46. Jayaraman, G., S.D. Gadam and S.M. Cramer (1993), Ion-Exchange Displacement Chromatography of Proteins: Dextran Based Polyelectrolytes as High Affinity Displacers, Journal of Chromatography A, 630, 53 - 68

  47. Gadam, S.D., G. Jayaraman and S.M. Cramer (1993), Characterization of Non-Linear Adsorption Properties of Dextran Based Polyelectrolyte Displacers in Ion - Exchange Systems, Journal of Chromatography A, 630, 37-52

  48. Subramanian, G., M. W. Phillips, G. Jayaraman and S. M. Cramer (1989), Displacement Chromatography of Biomolecules in Large Particle Diameter Systems, Journal of Chromatography A, 484, 225 - 236

  • Indicates co-first authors or co-corresponding authors