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Centres and Facilities

Flow Cytometry Facility

Model : FACS Aria III and FACS Calibur

Brand : BD

Flow cytometry is a technology that helps in the analysis of single cells in suspension. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals that are read by detectors such as photodiodes or photomultiplier tubes. These signals are converted into electronic signals that are analyzed by a computer and written to a standardized format (.fcs) data file. Cell populations can be analyzed and/or purified based on their fluorescent or light scattering characteristics.

Our departmental Flow Cytometry facility is equipped with one BD FACS ARIA III (Sorter) and one BD FACS Calibur (analyser). The BD FACS ARIA III and BD FACS Calibur is located at room no 419B of block 1. The facility provides technical personnel for consultation in experimental design and data interpretation. Offline data analysis can also be done using FloJoTM and DivaTM softwares through the offline analysis system of the facility. The facility conducts periodical training and workshops to educate young researchers.

Instrument Details

BD FACS ARIA III

The BD FACS ARIA III has 6 lasers and can detect 16 fluorochromes. The lasers available are as follows:

  • 375nm - Near UV
  • 405nm - Violet
  • 445nm - Purple
  • 488nm - Blue
  • 561nm - Yellow green
  • 633 nm - Red

BD FACS Calibur

The BD FACS Calibur has 2 lasers and can detect 4 fluorochromes. The lasers available are as follows:-

  • 488nm - Blue
  • 635nm - Red

Booking of Flow cytometer

Users can book the slots by sending email to facsiitm@smail.iitm.ac.in.

During acquisition, users have to provide the offline Requisition Form approved by the Principal Investigator and the Facility-in-Charge. Internal users have to submit the C-5 form along with the requisition forms.

BD FACS CALIBUR Requisition Form (Click here)

BD FACS ARIA III Requisition Form (Click here)

IC&SR Form C-5 (Click here) New C-5 form to be uploaded

Instrument Type of study BT internal users Academic Non-academic
BD FACS Calibur Analysis 100/sample 220* x No. of samples 440* x No. of samples
BD FACS Aria III sorter Analysis 100/sample 440* x No. of samples 660* x No. of samples
BD FACS Aria III sorter Sorting 500/sample 2200*/sample 5500*/sample

No blood samples and radioactive samples will be analysed.

The Departmental flow cytometry facility will be available only for acquiring the samples. Samples should be prepared carefully by the users as a single cell suspension and should be brought to the facility in person.

Users from other departments of IIT Madras will be treated as external academic samples; however, no service tax and overhead charges will be applicable to them. External academic /non-academic users have to pay as per the rates specified above along with GST @18%.

  • – Includes overhead charges of 10%

[Internal Users: For analysis of primary cells, attach Ethical Committee approval certificate, duly certified by the Principal Investigator that the samples are not infectious] Any further queries pertaining to the facility can be handled from 9.00 am to 5.00 pm on weekdays.

Please send mail to facsiitm@smail.iitm.ac.in or contact 044 22575138 for more details.

Persons Incharge: Srividhya R
email: facs@smail.iitm.ac.in
phone: 044 2257 5105