Our departmental Flow Cytometry facility is equipped with one BD FACS ARIA III (Sorter) and one BD FACS Calibur (analyser). The BD FACS ARIA III and BD FACS Calibur is located at room no 419B of block 1. The facility provides technical personnel for consultation in experimental design and data interpretation. Offline data analysis can also be done using FloJoTM and DivaTM softwares through the offline analysis system of the facility. The facility conducts periodical training and workshops to educate young researchers.
Instrument Details
BD FACS ARIA III
The BD FACS ARIA III has 6 lasers and can detect 16 fluorochromes. The lasers available are as follows:
- 375nm - Near UV
- 405nm - Violet
- 445nm - Purple
- 488nm - Blue
- 561nm - Yellow green
- 633 nm - Red
BD FACS Calibur
The BD FACS Calibur has 2 lasers and can detect 4 fluorochromes. The lasers available are as follows:-
- 488nm - Blue
- 635nm - Red
Booking of Flow cytometer
Users can book the slots by sending email to facsiitm@smail.iitm.ac.in.
During acquisition, users have to provide the offline Requisition Form approved by the Principal Investigator and the Facility-in-Charge. Internal users have to submit the C-5 form along with the requisition forms.
BD FACS CALIBUR Requisition Form (Click here)
BD FACS ARIA III Requisition Form (Click here)
IC&SR Form C-5 (Click here) New C-5 form to be uploaded
| Instrument | Type of study | BT internal users | Academic | Non-academic |
|---|---|---|---|---|
| BD FACS Calibur | Analysis | 100/sample | 220* x No. of samples | 440* x No. of samples |
| BD FACS Aria III sorter | Analysis | 100/sample | 440* x No. of samples | 660* x No. of samples |
| BD FACS Aria III sorter | Sorting | 500/sample | 2200*/sample | 5500*/sample |
No blood samples and radioactive samples will be analysed.
The Departmental flow cytometry facility will be available only for acquiring the samples. Samples should be prepared carefully by the users as a single cell suspension and should be brought to the facility in person.
Users from other departments of IIT Madras will be treated as external academic samples; however, no service tax and overhead charges will be applicable to them. External academic /non-academic users have to pay as per the rates specified above along with GST @18%.
- – Includes overhead charges of 10%
[Internal Users: For analysis of primary cells, attach Ethical Committee approval certificate, duly certified by the Principal Investigator that the samples are not infectious] Any further queries pertaining to the facility can be handled from 9.00 am to 5.00 pm on weekdays.
Please send mail to facsiitm@smail.iitm.ac.in or contact 044 22575138 for more details.