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Centres and Facilities

LCMS

Model : Orbitrap Elite

Brand : Thermo Fisher Scientific

Chromatography and Mass Spectrometry-Orbitrap Elite: Thermo Scientific Orbitrap Elite Mass spectrometer has a dual pressure linear ion trap coupled with high-field Orbitrap Mass analyzer facilitating increased sensitivity, reliability and dynamic range for better detection and MSn identification.

Features:

  • Novel high-field Orbitrap analyzer
  • Resolving power > 240,000 FWHM
  • Advanced signal processing
  • Multiple fragmentation techniques: CID, HCD and optionally ETD
  • Parallel MS and MSn analysis
  • Velos Pro ion trap generation II ion optics with neutral blocking technology increase robustness

Small Molecules:

Molecules ranging from 100 to 2000 Da can be analyzed for their presence through direct infusion method with an accuracy of < 5 ppm in high resolution mode.

Service Charge in INR Internal External Academic External Industries
300 1000+18%(Service Tax)* 3000+18%(Service Tax)
  • (Small Molecules Form for Internal):https://biotech.iitm.ac.in/Internal_Mass form_Small Molecules.doc
  • Small Molecules Form for Internal : link

Top Down Proteomics:

This involves identification of proteins present in a complex mixture prior digestion. Proteins extracted from the sample of interest and fractionated can be analysed using an Orbitrap based Mass spectrometer with CID, HCD and ETD fragmentation.

Service Charge in INR Internal External Academic External Industries
1000 10000+18%(Service Tax)* 20000+18%(Service Tax)

Bottom Up Proteomics:

Digested Protein samples can be analysed by LC-MS/MS. Peptides are separated using a nano LC-Column and the eluant is subjected to Mass spectrometer using Electrospray ionization.

Service Charge in INR Internal External Academic External Industries
1000 10000+18%(Service Tax)* 20000+18%(Service Tax)

Targeted Quantification:

Mass of the target is monitored in the MS1 level and MS/MS scans can be triggered on confirmation of the target.

Service Charge in INR Internal External Academic External Industries
1000 10000+18%(Service Tax)* 20000+18%(Service Tax)

Intact Protein Characterization:

Purified intact proteins can be characterized by top down methodology in an Orbitrap Elite Mass spectrometer by either direct infusion or LC coupled to an ESI source.

Service Charge in INR Internal External Academic External Industries
1000 10000+18%(Service Tax)* 20000+18%(Service Tax)
  • Form for Internal : link
  • Form for External : link
For sample submission mail to: lcms@smail.iitm.ac.in
Person in charge: Sasirekha M
email: lcms@smail.iitm.ac.in
phone: 044 2257 5105/5096